Clinical Information
Defense Clinical Report
This letter is to certify and validate properties pertaining to "Defense" brand anti-dermatophyte (ringworm) and anti-bacterial soap. Evidence is presented for the recognition of these properties.
Materials and Methods
LyfoCults brand dermatophyte ATCC (American Type Culture Collection) #28188 Trichophyton rubrum and a viable strain of Escherichia coli were the organisms utilized. Troy Biologicals brand DTM plates lot # 6065000 with exp. date 5/16/06 were utilized for Trichophyton rubrum along with BBL brand TSA II 5% SB agar plates lot # 6067642 with exp. date 7/4/06 were utilized for Escherichia coli. B. Braun Medical Inc. brand saline was utilized for reconstitution of Trichophyton rubrum.
Trichophyton rubrum was reconstituted from lyopholized (freeze-dried) form to a pasty consistancy. This consistancy was then plated onto the DTM plates using sterile swabs after a 10 minute incubation period. Plates were then incubated at room temperature for 7-10 days at which point luxurious growth became apparent. This step was performed to validate the viability of our strain of Trichophyton rubrum.
The growth was subcultured onto fresh DTM plates. Within 30 minutes of inoculating the DTM plates, a human test subject washed their hands with Defense brand soap, capturing the water falling from their hands into a sterile self-contained cup. A sterile pipette was used to transfer the solution onto the freshly inoculated DTM plates by placing drops in specifics areas onto the DTM plates. A yellow wax pencil was utilized to mark the three circles where the drops of soapy water drops fell. A total of four plates were utilized.
The viable strain of Escherichia coli was utilized by inoculating TSA plates with a lawn of Escherichia coli. Within minutes of inoculating the four BAP plates, drops of soapy water containing Defense brand soap. A yellow was pencil was utlilized to indicate the exact areas on the TSA plates by circling the three inoculation areas.
Results
Within nine days, growth became apparent over the entire area of the inoculated plates except in the yellow indicated cirlces where the soapy water drops were noted. This occured on all four DTM plates and the four TSA plates. Zones of inhibition were clearly present.
Discussion
Defense soap with tea tree oil presents visible anti-dermatophitic as well as anti-bacterial properties as evidenced by zones of inhibition (areas of no growth).
Erhardt Bell, M.T.President, PetLabs Diagnostic Laboratories, Inc
Diagnostic Laboratories
2510 Substation Rd., Medina, OH 44256
(330) 220-6435
Thursday, April 27, 2006
- Defense Soap Wipes
- Defense Soap Bars
- Defense Soap Gel
- Defense Soap Sanitizer
- Defense Soap Essential Oil
- Defense Soap Healing Salve
No time to hit the showers between matches? Defense Body Wipes are the perfect solution when you can’t hit the shower. Designed to be portable, you can take them on the road; leave a pack in your car, in your locker or anywhere else you need to be Defended!
CONTAINS NO PETROCHEMICAL COMPOUNDS, TRICLOSAN OR SODIUM LAURYL SULFATE. NO HARMFUL SIDE EFECTS.
Defense Soap Bars and the Defense Soap line of products are created specifically for combat athletes and anyone interested in using our all natural soap to keep their skin clean. Made with natural botanicals and only the finest natural essential oils, these products will keep you clean on and off the mat.
CONTAINS NO PETROCHEMICAL COMPOUNDS, TRICLOSAN OR SODIUM LAURYL SULFATE. NO HARMFUL SIDE EFFECTS.
Do you prefer shower gels to bar soaps? If so the Defense Shower Gel is perfect for you. All of the Defense Soap products are 100% Natural and contain active ingredients that have been clinically proven to have anti-fungal, antibacterial and antiviral properties.
CONTAINS NO PETROCHEMICAL COMPOUNDS, TRICLOSAN OR SODIUM LAURYL SULFATE. NO HARMFUL SIDE EFFECTS.
Defense Soap's "Bacteria Buster" sanitizer is the only equipment spray to use all natural ingredients. These pure pharmaceutical grade essential oils have been clinically proven to be effective against harmful bacteria, viruses and fungus. Simply fill the powerful 32 oz dispenser with ordinary tap water, mixing with our essential oil blend to create Defense Equipment Spray. Then spray and wipe. That's all you have to do to keep your equipment clean and fresh. Defense Equipment Spray has been developed for wall mats, heavy bags, and all other equipment that combat athlete's use while training. Defense Equipment Spray is NOT intended for walking surfaces including wrestling mats.
CONTAINS NO PETROCHEMICAL COMPOUNDS, TRICLOSAN OR SODIUM LAURYL SULFATE. NO HARMFUL SIDE EFFECTS.
Defense Pure pharmaceutical grade Essential Oils are a great way to treat skin infections naturally. By providing a moisturizer filled with pure ingredients that are beneficial to your skin, and help protect you from bacteria, viruses, and fungus both on and off the mat.
CONTAINS NO PETROCHEMICAL COMPOUNDS, TRICLOSAN OR SODIUM LAURYL SULFATE. NO HARMFUL SIDE EFFECTS.
The Defense Soap™ Healing Salve (1 oz.), is loaded with healing properties including vitamins A and E. This salve should be used in conjunction with the Defense Essential Oil, which will knock out ringworm and herpes sores quickly, but may leave the skin dry. The active ingredients (Tea Tree Oil and Oil of Eucalyptus) have been clinically proven to have anti-fungal, antibacterial and antiviral properties. The healing salve may also be used alone to aid in healing of mat burns and small scrapes and scratches suffered in practice.
CONTAINS NO PETROCHEMICAL COMPOUNDS, TRICLOSAN OR SODIUM LAURYL SULFATE. NO HARMFUL SIDE EFFECTS.
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